The standards for recycling stormwater are higher for drinking water than for non-potable reuse such as agricultural or urban irrigation. The Australian Drinking Water Guidelines (ADWG) inform regulations that ensure the removal of infectious pathogens and polluting chemicals from potable water, whereas the Australian Guidelines for Water Recycling (AGWR) ensure that non-potable recycled water does not pose a risk to human health. Compliance with these Guidelines often requires quantitative risk assessment of stormwater catchments, but this is an expensive and resource-intensive process. This research developed a ‘Chemical Hazard Assessment of Stormwater Micropollutants’ (CHASM) desktop tool to assess the suitability of stormwater for various potable and non-potable uses before commencing an expensive risk assessment, and to guide design of optimal and targeted monitoring and measuring programmes for chemicals of concern in any given catchment. Basic information about each of four Australian stormwater catchments (including size, land-use, and surface types) was entered into CHASM Excel spreadsheets. The tool utilises a database to generate a list of likely pollutants for that catchment, and optimal locations and times for monitoring. The CHASM tool proved reliable and easy to use.

Stormwater and treated wastewater can contain infectious pathogens. The Australian Guidelines for Water Recycling (AGWR) require that these are removed during recycling, and that recycling processes pass “Quantitative Microbial Risk Assessments” (QMRA). The problem is that these QMRA’s are often based on estimates. This research further developed methods (begun in WaterRA project 3002) to generate real-world measurements and data relevant to firefighters and domestic users because there are concerns that small amounts of recycled non-potable water might be inadvertently ingested. To test this, a harmless chemical, cyanuric acid, was added to safe water, then twenty-six volunteers used it, and a domestic high-pressure sprayer, to clean a full-sized model car for 10 minutes. The volunteers then collected their own urine for the next 24h because if this test-water is ingested, the cyanuric acid can be measured in the urine. From this it was discovered that the volunteers ‘drank’ an average of 0.13mL. This led to the conclusion that the conservative estimates in the AGWR currently protect domestic non-potable recycled water users, but that prolonged and/or high intensity occupational use of high-pressure sprays should be investigated further.

Compliance with the Australian Guidelines for Water Recycling ensures that recycled wastewater does not present a health risk due to infectious pathogens or disease-causing chemicals. Many pathogens in wastewater are inactivated by disinfection treatments such as chlorination, but this causes a problem when disinfectants react with nitrogen compounds in wastewater and form Disinfection By-Products (DBPs), some of which pose a health risk. This research collected samples from four wastewater treatment plants (WWTPs) with different treatment methods and climate zones. A comprehensive and innovative analysis of the types of pathogens, various chemical forms of nitrogen and DBPs, and removal of these components during the recycling process, was related to season, climate and the four different treatment trains. It was concluded that the WWTP using a combined anaerobic/aerobic pond system was best at removing nitrogens and minimising DBP formation, but the best overall treatment performance was delivered by an activated sludge, oxidation ditch and infiltration pond WWTP in a temperate climate. Pathogens were found in influents but not treated effluents, and so were other nitrogen-removing micro-organisms. Treatment was better in summer, and the wastewater quality in these four WWTPs posed a low health and environmental risk to non-potable reuse.

The Australian Guidelines for Water Recycling (AGWR) require water recycling treatment processes to be validated in ways that ensure that recycled water does not pose a risk to health, safety or the environment. Stage I of this project developed a National Validation Framework (WaterRA project 3009). This Stage II develops and describes detailed protocols that utilities can apply to implement the validation framework and achieve compliance with the AGWR.

Recycling wastewater by using reverse osmosis (RO) and ultrafiltration appears to be associated with the formation of some groups of micropollutants but there is not much information about these processes. This research selected iodinated disinfection by-products (DBPs) and N-nitrosamines (NDMA), and benzotriazoles and benzothiazoles, which are compounds in dishwasher detergents, for further investigation. It was concluded that minimising the formation of dichloramine (a precursor molecule to NDMA formation) by reducing pH and maximising activated sludge ammonia production, reduced the formation of N-nitrosamines in RO-treated wastewater. Iodinated DBPs and benzotriazoles were detected in RO treated wastewater in this study but at lower concentrations than those thought to pose a risk to human health.

Membranes are used to remove viruses from treated wastewater to make it safe for discharge or recycling. It is important to monitor the integrity of membranes and check that viruses cannot break through. This creates a need for surrogates that are cheaper, safer and easier to use than the non-hazardous indicator virus currently applied to test membrane integrity. This research synthesised a series of fluorescent nanoparticles with similar dimensions to viruses, with the idea that the fluorescence would support continuous in situ monitoring in real time, but unfortunately the nanoparticles did not emit enough fluorescence and could not be detected in the product water.

Smaller and regional Wastewater Treatment Plants (WWTPs) have the capacity to recycle wastewater for agricultural use, but the cost of obtaining regulatory approval or ‘accreditation’ is prohibitive. One reason for this is that each WWTP must demonstrate that its processes and operations consistently remove pathogens that cause infectious diseases in humans. Operating conditions include flow rate through the WWTP, and temperature in the activated sludge component of the WWTPs. Although pathogen ‘log removal values (LVR)’ were obtained for a WWTP at 19-20°C in Part I of this project (WQRA project 2001), these values cannot also be attributed to summer temperatures of 26°C. This research determined LRVs for ‘new’ WWTP operating conditions and combined the data with data from Phase I (Project 2001) for analysis. One of the conclusions from Part II was that faster flow rates associated with increased rainfall reduced pathogen LRVs.

The Australian water industry uses a variety of membrane processes to remove unwanted pathogens or compounds, such as salt, from source waters. As membranes age their ability to fulfil these removal and filtering functions declines. The problem is that although there are recognised and validated tests for membrane integrity, they are usually performed only on new membranes, and information about the effect of membrane aging on pathogen removal is limited. This project reviewed published literature and identified four methods suitable for future development as Membrane Integrity Tests that may prove applicable to evaluate aging membranes during ongoing and long-term plant operation.

Wastewater must be treated to remove harmful pathogens and chemicals before it can be released to the environment, but the cost of proving that all pollutants have been removed is prohibitive because potentially thousands of separate chemicals would have to be measured. Another problem is that classical chemistry measurement tests are sometimes not sensitive enough to be able to detect the very low levels of chemicals which still harm animals and plants. This research developed a suite of extremely sensitive in vitro cell culture tests and an in-situ laboratory test in which mosquitofish were observed when swimming in recycled treated water. These bioassays measured the effects of mixtures of contaminants and were compared with traditional chemical measurements of separate contaminants. The in vitro cell culture, in situ mosquitofish and classical chemical analyses of selected contaminants generated equivalent results and led to the conclusion that combining multiple lines of evidence into a toolbox approach for the assessment of water quality provides data which is more informative and relevant when assessing potential impacts on the environment than traditional chemical measurements alone.

Wastewater (WW) contains harmful chemicals, including pesticides, that can disrupt normal gene function or hormone activity. The cost of measuring each separate contaminant at the frequency needed to demonstrate the safety of recycled WW is prohibitive. This research reviewed the risk assessment and regulation of chemicals in Australian water, with a focus on ‘thresholds of toxicological concern’. Laboratory techniques were developed to extract and concentrate WW contaminants into solutions suitable for analysis using both new in vitro cell culture assays and analysis in expensive, established chemical tests. WW and treated samples were collected from nine Australian water reclamation plants. The total effect of each sample (which contained a mixture of contaminants) on cell death, gene integrity and aspects of liver, hormone, nerve and immune system activity, was determined using in vitro cell culture bioassays, and compared with the classical chemical measurement of each separate contaminant. The cheaper cell-culture tests correlated well to the levels of groups of chemicals and could be used to find thresholds of toxicological concern. Both testing regimens also demonstrated that reverse osmosis is a highly effective method that removes harmful chemicals to levels much lower than those designated safe by regulatory authorities.