European carp have decimated native fish species in the Murray-Darling River. The federally funded National Carp Control Plan proposes using a carp-specific virus to kill the pest-fish, but before doing so are consulting with a broad array of environmental, conservation and other stakeholders, including the water industry. There are concerns that large amounts of dead and decaying carp near water offtakes or storages might overwhelm the capacity of water treatment plants (WTPs) to remove organic matter and taste and odour compounds, and might compromise the production of safe, palatable drinking water. In this research a series of experiments led to the conclusions that medium to high carp densities could be managed by adding a 30 minute procedure to existing WTP methods, and that there would not be an increased risk to public health.

E. coli bacteria naturally populate the gastrointestinal tract of humans and animals; they are usually harmless and are commonly excreted. Faeces can also contain harmful microscopic pathogens, and this has led to the assumption that if harmless E. coli are found in water, that the drinking water has been contaminated with faeces that might also have contained pathogens that pose a risk to public health. Using E. coli as an indicator of faecal contamination was recently challenged by the finding that some E. coli strains live, grow and bloom in the environment, and their presence in water might not mean that the water has been contaminated with harmful pathogens. This research examined the environmental conditions associated with E. coli bloom formation in the context of climate-change adaptation and developed multiplex PCR tests which allow the identification of environmental and faecal E. coli. This information was added to a Utility Response Protocol.

Disinfection is essential for removing harmful microbial pathogens and making safe drinking water but can also cause formation of disinfection by-products (DBPs), some of which pose a health risk. Thirty years of research have amassed a wealth of knowledge about the identification, formation, treatment and control, toxicology and epidemiology of DBPs in Australia. This project compiled, assessed and presented an overview of DBP-related research in Australia.

The ADWG has methods for predicting risks to water quality, but these were not developed for managing extreme climate-change driven weather events such as bushfires or floods. This research developed a risk assessment tool for managing water-related health risks associated with extreme weather events. Real-world datasets and experience of water cloudiness (turbidity), colour and blue-green algae were used to create and validate environmental models which were developed further by applying Baysian network and System Dynamics concepts. This iteration of the model was not constrained by, and did not reflect existing risk profiles, but was judged to be flexible enough to provide a realistic representation of future hazards arising from extreme weather events.

Microscopic pathogens in drinking water pose a risk to public health. In Australia, chlorine or chloramine are used to inactivate these pathogens, to disinfect drinking water and prevent widespread outbreaks of debilitating illness in large populations. Although largely successful, problems arise when drinking water is contaminated by pathogens after being disinfected in the treatment plant but before reaching the customer. A variety of situations cause this, one being small pipe leaks which become a route for soil micro-organisms to get into drinking water. To prevent pathogen contamination in pipe networks, higher levels of disinfectants can be added at the treatment plant, or secondary disinfection can be administered in the pipe network. This research project produced a guidance manual which explains how to maintain and monitor effective disinfection levels in post-treatment pipelines, and the major challenges to maintaining effective disinfection.

The ADWGs include methods and strategies for calculating all types of risks to water quality but lack guidance for managing extreme weather events. This research completed a number of activities which included the collection and analysis of 10 Australian extreme weather events and listed transferrable, real-world lessons and experiences. Two hypothetical scenarios based on extreme flood, and on bushfire combined with significant rainfall, were designed then ‘managed’ by diverse water utility personnel and health regulators. This activity also generated a list of key lessons. Optimal methods for calculating short-term Exposure Trigger Values for hazardous compounds were identified. Outcomes from all the project activities were processed and collated into a practical instruction guide to support Australian water utilities in their management of extreme weather events.

Cryptosporidium, a microscopic pathogen, forms infectious oocysts which are removed by specific and targeted water treatments. Oocysts can only be seen by using a microscope but finding an infectious dose of 10 oocysts in a litre of water is like finding a needle in a haystack. Usually a larger volume of water, 1000mL, is filtered to recover all the oocysts into a small volume of 0.1 to 1 mL, because this is small enough to be examined under a microscope. It is scientific practice to add some dead, colour-dyed oocysts to the large volume of water. If all the coloured oocysts are counted on the filter, the recovery is 100%. From this it became clear that there was a problem with oocyst recovery. This research found that different elements reduced recovery from different types of water, for example, iron and silica reduced oocyst recovery from river or groundwaters. The approved method for quantifying the environmental occurrence of oocysts can now be modified to increase recovery, and this change improves analysis and consequent management which further reduces risks to public health.

Source waters are disinfected to remove harmful pathogens, but chlorine reacts with organic matter and bromides to form disinfection by-products (DBPs) which can affect health. Water treatment reduces DBPs to safe levels by using alum to remove organic matter before disinfection but some water sources, particularly those with high bromine levels, are still difficult to treat. This research aimed to compile the best protocols for alum coagulation and disinfection when source waters contain different levels of organic matter and bromides, and to relate these to health risks. When organic matter was removed with 125 mg/L alum, and this treated source water was disinfected twice, and the first dose was calculated to generate chlorine levels of 0.5 mg / L for two days before administering the second dose, then DBP levels in drinking water were minimised. Neither alum-treated nor disinfected water caused toxicity in two laboratory tests used to examine risks to health.

Components of dissolved organic matter (DOM) and dissolved organic nitrogen (DON) in source waters can react with disinfecting chlorine or chloramine to form nitrogenous disinfection byproducts (n-DBPs) which might be toxic and hazardous to health. In this research, water samples were collected from nine water treatment plants and found to contain 28 n-DBPs. Total n-DBP formation, and particularly brominated n-DBP formation, was affected more by the levels of bromine in raw water than the different forms of nitrogen, and this led to the recommendation that it could be beneficial to monitor raw waters with high bromine concentrations. Although chloramination caused formation of more n-DBPs than chlorination, coagulation treatment decreased total DBP levels. Further research was recommended to characterise the toxicity of n-DBPs and to optimise the removal of DOM, DON and other n-DBP precursors by using GAC Acticarb in the treatment train.

Cryptosporidium, a microscopic single-cell parasite, forms an “oocyst” with a resistant outer layer analogous to an eggshell. Oocysts survive for a long time in the environment but UV in sunlight, and high temperatures that cause desiccation, kill them. If a mammal drinks water containing live oocysts, they embed in the gut wall and continue their lifecycle until eventually many more oocysts are excreted. There are 26 species of cryptosporidium but only five infect humans, and two; Cryptosporidium hominus and Cryptosporidium parvum, cause approximately 95% of all human infections. C. hominus occurs only in humans, but C. parvum is also found in cattle, sheep, and other animals. The problem is that human-infecting oocysts are excreted by animals in catchments and rain can wash live oocysts into water reservoirs. This research collated peer-reviewed published literature, and information and data collected by the water industry, then characterised the distribution of different Cryptosporidium species in Australian catchments. This led to recognition of a research need to track and predict live and dead oocyst transport during different weather events, and to model and evaluate catchment management initiatives such as excluding cattle from reservoir areas.